Open AccessEfficient multi-site two-photon functional imaging of neuronal circuits
Author(s) -
Michael Lawrence Castañares,
Vini Gautam,
J. C. Drury,
Hans Bachor,
Vincent Ricardo Daria
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.005325
Subject(s) - photon , calcium imaging , fluorescence , optics , detector , gating , photon counting , physics , signal to noise ratio (imaging) , two photon excitation microscopy , signal (programming language) , hippocampal formation , optical imaging , optoelectronics , computer science , materials science , biophysics , calcium , neuroscience , programming language , metallurgy , biology
Two-photon imaging using high-speed multi-channel detectors is a promising approach for optical recording of cellular membrane dynamics at multiple sites. A main bottleneck of this technique is the limited number of photons captured within a short exposure time (~1ms). Here, we implement temporal gating to improve the two-photon fluorescence yield from holographically projected multiple foci whilst maintaining a biologically safe incident average power. We observed up to 6x improvement in the signal-to-noise ratio (SNR) in Fluorescein and cultured hippocampal neurons showing evoked calcium transients. With improved SNR, we could pave the way to achieving multi-site optical recording of fluorogenic probes with response times in the order of ~1ms.