Open AccessDepth-resolved imaging of colon tumor using optical coherence tomography and fluorescence laminar optical tomography
Author(s) -
Qinggong Tang,
Jianting Wang,
Aaron Frank,
Jonathan Lin,
Zhifang Li,
Chao-Wei Chen,
Lily Jin,
Tongning Wu,
Bruce D. Greenwald,
Hiroshi Mashimo,
Yu Chen
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.005218
Subject(s) - optical coherence tomography , optical tomography , tomography , diffuse optical imaging , molecular imaging , fluorescence lifetime imaging microscopy , materials science , optics , gold standard (test) , biomedical engineering , medicine , radiology , fluorescence , in vivo , physics , biology , microbiology and biotechnology
Early detection of neoplastic changes remains a critical challenge in clinical cancer diagnosis and treatment. Many cancers arise from epithelial layers such as those of the gastrointestinal (GI) tract. Current standard endoscopic technology is difficult to detect the subsurface lesions. In this research, we investigated the feasibility of a novel multi-modal optical imaging approach including high-resolution optical coherence tomography (OCT) and high-sensitivity fluorescence laminar optical tomography (FLOT) for structural and molecular imaging. The C57BL/6J-Apc Min /J mice were imaged using OCT and FLOT, and the correlated histopathological diagnosis was obtained. Quantitative structural (scattering coefficient) and molecular (relative enzyme activity) parameters were obtained from OCT and FLOT images for multi-parametric analysis. This multi-modal imaging method has demonstrated the feasibility for more accurate diagnosis with 88.23% (82.35%) for sensitivity (specificity) compared to either modality alone. This study suggested that combining OCT and FLOT is promising for subsurface cancer detection, diagnosis, and characterization.