Open AccessVolumetric optical mapping in early embryonic hearts using light-sheet microscopy
Author(s) -
Pei Ma,
Dennis Chan,
Shi Gu,
Michiko Watanabe,
Michael W. Jenkins,
Andrew M. Rollins
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.005120
Subject(s) - optical mapping , heartbeat , embryonic heart , light sheet fluorescence microscopy , microscopy , voltage sensitive dye , optical microscope , materials science , optics , biomedical engineering , orientation (vector space) , fluorescence microscope , fluorescence , computer science , optical imaging , embryonic stem cell , chemistry , physics , biology , medicine , mathematics , biochemistry , genetics , computer security , geometry , gene , scanning electron microscope
Optical mapping (OM) of electrical activity using voltage-sensitive fluorescent dyes is a powerful tool for the investigation of embryonic cardiac electrophysiology. However, because conventional OM integrates the signal in depth and projects it to a two-dimensional plane, information acquired is incomplete and dependent upon the orientation of the sample. This complicates interpretation of data, especially when comparing one heart to another. To overcome this limitation, we present volumetric OM using light-sheet microscopy, which enables high-speed capture of optically sectioned slices. Voltage-sensitive fluorescence images from multiple planes across entire early embryonic quail hearts were acquired, and complete, orientation-independent, four-dimensional maps of transmembrane potential are demonstrated. Volumetric OM data were collected while using optical pacing to control the heart rate, paving the way for physiological measurements and precise manipulation of the heartbeat in the future.