Open AccessRegion-based diffuse optical tomography with registered atlas: in vivo acquisition of mouse optical properties
Author(s) -
Wenbo Wan,
Yihan Wang,
Jun Qi,
Lingling Liu,
Wenjuan Ma,
Jiao Li,
Limin Zhang,
Zhongxing Zhou,
Huijuan Zhao,
Feng Gao
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.005066
Subject(s) - diffuse optical imaging , a priori and a posteriori , optical tomography , atlas (anatomy) , tomography , computer science , segmentation , computer vision , optical imaging , optical coherence tomography , preclinical imaging , artificial intelligence , biomedical engineering , iterative reconstruction , optics , in vivo , physics , medicine , anatomy , biology , philosophy , microbiology and biotechnology , epistemology
The reconstruction quality in the model-based optical tomography modalities can greatly benefit from a priori information of accurate tissue optical properties, which are difficult to be obtained in vivo with a conventional diffuse optical tomography (DOT) system alone. One of the solutions is to apply a priori anatomical structures obtained with anatomical imaging systems such as X-ray computed tomography (XCT) to constrain the reconstruction process of DOT. However, since X-ray offers low soft-tissue contrast, segmentation of abdominal organs from sole XCT images can be problematic. In order to overcome the challenges, the current study proposes a novel method of recovering a priori organ-oriented tissue optical properties, where anatomical structures of an in vivo mouse are approximately obtained by registering a standard anatomical atlas, i.e. , the Digimouse, to the target XCT volume with the non-rigid image registration, and, in turn, employed to guide DOT for extracting the optical properties of inner organs. Simulative investigations have validated the methodological availability of such atlas-registration-based DOT strategy in revealing both a priori anatomical structures and optical properties. Further experiments have demonstrated the feasibility of the proposed method for acquiring the organ-oriented tissue optical properties of in vivo mice, making it as an efficient way of the reconstruction enhancement.