Open AccessAdaptive illumination reduces photobleaching in structured illumination microscopy
Author(s) -
Nadya Chakrova,
Alicia Soler Canton,
Christophe Danelon,
Sjoerd Stallinga,
Bernd Rieger
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.004263
Subject(s) - photobleaching , microscopy , optics , fluorescence recovery after photobleaching , microscope , digital holographic microscopy , fluorescence microscope , materials science , fluorescence , computer vision , computer science , physics
Photobleaching is a major factor limiting the observation time in fluorescence microscopy. We achieve photobleaching reduction in structured illumination microscopy (SIM) by locally adjusting the illumination intensities according to the sample. Adaptive SIM is enabled by a digital micro-mirror device (DMD), which provides a projection of the grayscale illumination patterns. We demonstrate a reduction in photobleaching by a factor of three in adaptive SIM compared to the non-adaptive SIM based on a spot grid scanning approach. Our proof-of-principle experiments show great potential for DMD-based microscopes to become a more useful tool in live-cell SIM imaging.