Open Access3D imaging in CUBIC-cleared mouse heart tissue: going deeper
Author(s) -
Imke Nehrhoff,
Diana Bocancea,
Javier Vaquero,
Juan J. Vaquero,
Jorge Ripoll,
Manuel Desco,
Marı́a Victoria Gómez-Gaviro
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.003716
Subject(s) - clearance , microscope , biomedical engineering , penetration (warfare) , confocal , confocal laser scanning microscopy , image quality , confocal microscopy , penetration depth , computer science , materials science , pathology , medicine , artificial intelligence , optics , physics , image (mathematics) , operations research , engineering , urology
The ability to acquire high resolution 3D images of the heart enables to study heart diseases more in detail. In this work, the CUBIC (clear, unobstructed brain imaging cocktails and computational analysis) clearing protocol was optimized for thick mouse heart sections to enhance the penetration depth of the confocal microscope lasers into the tissue. In addition, the optimized CUBIC clearing of the heart enhances antibody penetration into the tissue by a factor of five. The present protocol enables deep 3D high-quality image acquisition in the heart allowing a much more accurate assessment of the cellular and structural changes that underlie heart diseases.