3D imaging in CUBIC-cleared mouse heart tissue: going deeper | Zendy

Imke Nehrhoff | Zendy; Diana I. Bocancea | Zendy; Javier Vaquero | Zendy; J.J. Vaquero | Zendy; Jorge Ripoll | Zendy; Manuel Desco | Zendy; María Victoria GómezGaviro | Zendy

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3D imaging in CUBIC-cleared mouse heart tissue: going deeper

Author(s) -

Imke Nehrhoff,

Diana I. Bocancea,

Javier Vaquero,

J.J. Vaquero,

Jorge Ripoll,

Manuel Desco,

María Victoria GómezGaviro

Publication year - 2016

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.7.003716

Subject(s) - clearance , microscope , biomedical engineering , penetration (warfare) , confocal , confocal laser scanning microscopy , image quality , confocal microscopy , penetration depth , computer science , materials science , pathology , medicine , artificial intelligence , optics , physics , image (mathematics) , operations research , engineering , urology

The ability to acquire high resolution 3D images of the heart enables to study heart diseases more in detail. In this work, the CUBIC (clear, unobstructed brain imaging cocktails and computational analysis) clearing protocol was optimized for thick mouse heart sections to enhance the penetration depth of the confocal microscope lasers into the tissue. In addition, the optimized CUBIC clearing of the heart enhances antibody penetration into the tissue by a factor of five. The present protocol enables deep 3D high-quality image acquisition in the heart allowing a much more accurate assessment of the cellular and structural changes that underlie heart diseases.

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