Open AccessHigh-resolution real-time dual-view imaging with multiple point of view microscopy
Author(s) -
Pierre Mangeol,
Erwin J. G. Peterman
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.003631
Subject(s) - computer science , microscopy , sample (material) , microscope , computer vision , point (geometry) , light sheet fluorescence microscopy , optics , point spread function , resolution (logic) , fluorescence microscope , artificial intelligence , scanning confocal electron microscopy , fluorescence , physics , mathematics , geometry , thermodynamics
Most methods to observe three-dimensional processes in living samples are based on imaging a single plane that is sequentially scanned through the sample. Sequential scanning is inherently slow, which can make it difficult to capture objects moving quickly in three dimensions. Here we present a novel method, multiple point-of-view microscopy (MPoVM), that allows simultaneous capturing of the front and side views of a sample with high resolution. MPoVM can be implemented in most fluorescence microscopes, offering new opportunities in the study of dynamic biological processes in three dimensions.