Open AccessImaging hemodynamic response after ischemic stroke in mouse cortex using visible-light optical coherence tomography
Author(s) -
Siyu Chen,
Qi Liu,
Shuangjiu Xiao,
Brian Soetikno,
Shanbao Tong,
Hao F. Zhang
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.003377
Subject(s) - penumbra , optical coherence tomography , diffuse optical imaging , hemodynamics , optics , biomedical engineering , optical tomography , medicine , ischemia , materials science , tomography , physics , cardiology
Visible-light optical coherence tomography (Vis-OCT) is an emerging technology that can image hemodynamic response in microvasculature. Vis-OCT can retrieve blood oxygen saturation (sO 2 ) mapping using intrinsic optical absorption contrast while providing high-resolution anatomical vascular structures at the same time. To improve the accuracy of Vis-OCT oximetry on vessels embedded in highly scattering medium, i.e., brain cortex, we developed and formulated a novel dual-depth sampling and normalization strategy that allowed us to minimize the detrimental effect of ubiquitous tissue scattering. We applied our newly developed approach to monitor the hemodynamic response in mouse cortex after focal photothrombosis. We observed vessel dilatation, which was negatively correlated with the original vessel diameter, in the penumbra region. The sO 2 of vessels in the penumbra region also dropped below normal range after focal ischemia.