Open AccessVisible-light optical coherence tomography-based multimodal retinal imaging for improvement of fluorescent intensity quantification
Author(s) -
Zahra Nafar,
Mawei Jiang,
Rong Wen,
Shuliang Jiao
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.003220
Subject(s) - optical coherence tomography , autofluorescence , retinal pigment epithelium , optics , retinal , fluorescence , materials science , fluorescence lifetime imaging microscopy , preclinical imaging , retina , imaging phantom , biomedical engineering , medicine , physics , ophthalmology , microbiology and biotechnology , biology , in vivo
We developed a spectral-domain visible-light optical coherence tomography (VIS-OCT) based multimodal imaging technique which can accomplish simultaneous OCT and fluorescence imaging with a single broadband light source. Phantom experiments showed that by using the simultaneously acquired OCT images as a reference, the effect of light attenuation on the intensity of the fluorescent images by materials in front of the fluorescent target can be compensated. This capability of the multimodal imaging technique is of high importance for achieving quantification of the true intensities of autofluorescence (AF) imaging of the retina. We applied the technique in retinal imaging including AF imaging of the retinal pigment epithelium and fluorescein angiography (FA). We successfully demonstrated the effect of compensation on AF and FA images with the simultaneously acquired VIS-OCT images.