Open AccessTomographic imaging of transparent biological samples using the pyramid phase microscope
Author(s) -
Ignacio Iglesias
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.003049
Subject(s) - microscope , optics , pyramid (geometry) , microscopy , refractive index , biological imaging , tomography , tomographic reconstruction , biological specimen , materials science , spatial frequency , physics , fluorescence
We show how a pyramid phase microscope can be used to obtain tomographic information of the spatial variation of refractive index in biological samples using the Radon transform. A method that uses the information provided by the phase microscope for axial and lateral repositioning of the sample when it rotates is also described. Its application to the reconstruction of mouse embryos in the blastocyst stage is demonstrated.