Open AccessFrequency domain photoacoustic and fluorescence microscopy
Author(s) -
Gregor Langer,
Bianca Buchegger,
Jaroslaw Jacak,
Thomas A. Klar,
Thomas Berer
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.002692
Subject(s) - photoacoustic doppler effect , optics , microscopy , fluorescence , materials science , photoacoustic imaging in biomedicine , fluorescence microscope , frequency domain , resolution (logic) , signal (programming language) , physics , computer science , artificial intelligence , computer vision , programming language
We report on simultaneous frequency domain optical-resolution photoacoustic and fluorescence microscopy with sub-µm lateral resolution. With the help of a blood smear, we show that photoacoustic and fluorescence images provide complementary information. Furthermore, we compare theoretically predicted signal-to-noise ratios of sinusoidal modulation in frequency domain with pulsed excitation in time domain.