Open AccessApplicability, usability, and limitations of murine embryonic imaging with optical coherence tomography and optical projection tomography
Author(s) -
Manmohan Singh,
Raksha Raghunathan,
Victor G. Piazza,
Anjul M. Davis-Loiacono,
Alex Cable,
Tegy J. Vedakkan,
Trevor Janecek,
Michael V. Frazier,
Achuth Nair,
Chen Wu,
Irina V. Larina,
Mary E. Dickinson,
Kirill V. Larin
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.002295
Subject(s) - optical coherence tomography , tomography , preclinical imaging , visualization , clearance , optical tomography , embryo , embryonic stem cell , optics , biomedical engineering , in vivo , computer science , biology , medicine , microbiology and biotechnology , artificial intelligence , physics , biochemistry , gene , urology
We present an analysis of imaging murine embryos at various embryonic developmental stages (embryonic day 9.5, 11.5, and 13.5) by optical coherence tomography (OCT) and optical projection tomography (OPT). We demonstrate that while OCT was capable of rapid high-resolution live 3D imaging, its limited penetration depth prevented visualization of deeper structures, particularly in later stage embryos. In contrast, OPT was able to image the whole embryos, but could not be used in vivo because the embryos must be fixed and cleared. Moreover, the fixation process significantly altered the embryo morphology, which was quantified by the volume of the eye-globes before and after fixation. All of these factors should be weighed when determining which imaging modality one should use to achieve particular goals of a study.