Open AccessOptically controlled release of DNA based on nonradiative relaxation process of quenchers
Author(s) -
Yusuke Ogura,
Atsushi Onishi,
Takahiro Nishimura,
Jun Tanida
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.002142
Subject(s) - fluorescence , relaxation (psychology) , dna , excitation , chemistry , photochemistry , substrate (aquarium) , biophysics , optoelectronics , materials science , optics , physics , psychology , social psychology , biochemistry , oceanography , quantum mechanics , biology , geology
Optically controlled release of a DNA strand based on a nonradiative relaxation process of black hole quenchers (BHQs), which are a sort of dark quenchers, is presented. BHQs act as efficient energy sources because they relax completely via a nonradiative process, i.e., without fluorescent emission-based energy losses. A DNA strand is modified with BHQs and the release of its complementary strand is controlled by excitation of the BHQs. Experimental results showed that up to 50% of the target strands were released, and these strands were capable of inducing subsequent reactions. The controlled release was localized on a substrate within an area of no more than 5 micrometers in diameter.