Open AccessDynamic full field optical coherence tomography: subcellular metabolic contrast revealed in tissues by interferometric signals temporal analysis
Author(s) -
Clément Apelian,
Fabrice Harms,
Olivier Thouvenin,
A. C. Boccara
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.001511
Subject(s) - optical coherence tomography , optics , interferometry , millisecond , coherence (philosophical gambling strategy) , dynamic range , contrast (vision) , tomography , physics , temporal resolution , biological system , biology , quantum mechanics , astronomy
We developed a new endogenous approach to reveal subcellular metabolic contrast in fresh ex vivo tissues taking advantage of the time dependence of the full field optical coherence tomography interferometric signals. This method reveals signals linked with local activity of the endogenous scattering elements which can reveal cells where other OCT-based techniques fail or need exogenous contrast agents. We benefit from the micrometric transverse resolution of full field OCT to image intracellular features. We used this time dependence to identify different dynamics at the millisecond scale on a wide range of organs in normal or pathological conditions.