Open AccessMature red blood cells: from optical model to inverse light-scattering problem
Author(s) -
Konstantin V. Gilev,
Maxim A. Yurkin,
Ekaterina S. Chernyshova,
Dmitry I. Strokotov,
Andrei V. Chernyshev,
Valeri P. Maltsev
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.001305
Subject(s) - refractive index , light scattering , optics , isotropy , materials science , scattering , curvature , elasticity (physics) , inverse problem , physics , mathematics , geometry , mathematical analysis , composite material
We propose a method for characterization of mature red blood cells (RBCs) morphology, based on measurement of light-scattering patterns (LSPs) of individual RBCs with the scanning flow cytometer and on solution of the inverse light-scattering (ILS) problem for each LSP. We considered a RBC shape model, corresponding to the minimal bending energy of the membrane with isotropic elasticity, and constructed an analytical approximation, which allows rapid simulation of the shape, given the diameter and minimal and maximal thicknesses. The ILS problem was solved by the nearest-neighbor interpolation using a preliminary calculated database of 250,000 theoretical LSPs. For each RBC in blood sample we determined three abovementioned shape characteristics and refractive index, which also allows us to calculate volume, surface area, sphericity index, spontaneous curvature, hemoglobin concentration and content.