Open AccessIn vivo quantifying molecular specificity of Cy55-labeled cyclic 9-mer peptide probe with dynamic fluorescence imaging
Author(s) -
Yunpeng Dai,
Jiaqi Yin,
Yu Huang,
Xueli Chen,
Guodong Wang,
Yajun Liu,
Xianghan Zhang,
Yongzhan Nie,
Kaichun Wu,
Jimin Liang
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.001149
Subject(s) - in vivo , fluorescence , molecular imaging , fluorescence lifetime imaging microscopy , chemistry , preclinical imaging , biomedical engineering , biological system , nuclear medicine , biology , physics , optics , medicine , microbiology and biotechnology
We quantified molecular specificity of Cy5.5-GX1 in vivo with dynamic fluorescence imaging to better understand its kinetic properties. According to whether or not free GX1 was injected and when it was injected, twelve of BGC-823 xenografted mice were randomly divided into three groups and underwent a 60 minute dynamic fluorescence scanning. Combined with a principal-component analysis, the binding potential (Bp) of the probe was determined by both Logan graphical analysis with reference tissue model (GARTM) and Lammertsma simplified reference tissue model (SRTM). The sum of the pharmacokinetic rate constants (SKRC) was quantified by the Gurfinkel exponential model (GEXPM). Cy5.5-GX1 specifically targeted tumor both in vitro and in vivo. We obtained similar quantification results of Bp (GARTM Bp = 0.582 ± 0.2655, SRTM Bp = 0.618 ± 0.2923), and obtained a good linear relation between the Bp value and the SKRC value. Our results indicate that the SKRC value is more suitable for an early-stage kinetic data analysis, and the Bp value depicts kinetic characteristics under the equilibrium state. Dynamic fluorescence imaging in conjunction with various kinetic models are optimal tools to quantify molecular specificity of the Cy5.5-GX1 probe in vivo.