Open AccessIn vivo bioluminescence and reflectance imaging of multiple organs in bioluminescence reporter mice by bundled-fiber-coupled microscopy
Author(s) -
Yumiko Ando,
Takashi Sakurai,
Kowa Koida,
Hajime Tei,
Akiko Hida,
Kazuki Nakao,
Mistuo Natsume,
Rika Numano
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.000963
Subject(s) - bioluminescence , bioluminescence imaging , preclinical imaging , microscopy , biomedical engineering , in vivo , microscope , optical fiber , optics , luciferase , materials science , biology , pathology , medicine , physics , ecology , transfection , genetics , microbiology and biotechnology , cell culture
Bioluminescence imaging (BLI) is used in biomedical research to monitor biological processes within living organisms. Recently, fiber bundles with high transmittance and density have been developed to detect low light with high resolution. Therefore, we have developed a bundled-fiber-coupled microscope with a highly sensitive cooled-CCD camera that enables the BLI of organs within the mouse body. This is the first report of in vivo BLI of the brain and multiple organs in luciferase-reporter mice using bundled-fiber optics. With reflectance imaging, the structures of blood vessels and organs can be seen clearly with light illumination, and it allowed identification of the structural details of bioluminescence images. This technique can also be applied to clinical diagnostics in a low invasive manner.