Open AccessTri-modal microscope for head and neck tissue identification
Author(s) -
Étienne De Montigny,
Nadir Goulamhoussen,
WendyJulie Madore,
Mathias Strupler,
Olguta Ecaterina Gologan,
Tareck Ayad,
Caroline Boudoux
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.000732
Subject(s) - optical coherence tomography , microscope , optics , microscopy , computer science , interferometry , light sheet fluorescence microscopy , biomedical engineering , medicine , scanning confocal electron microscopy , physics
A novel tri-modal microscope combining optical coherence tomography (OCT), spectrally encoded confocal microscopy (SECM) and fluorescence imaging is presented. This system aims at providing a tool for rapid identification of head and neck tissues during thyroid surgery. The development of a dual-wavelength polygon-based swept laser allows for synchronized, co-registered and simultaneous imaging with all three modalities. Further ameliorations towards miniaturization include a custom lens for optimal compromise between orthogonal imaging geometries as well as a double-clad fiber coupler for increased throughput. Image quality and co-registration is demonstrated on freshly excised swine head and neck tissue samples to illustrate the complementarity of the techniques for identifying signature cellular and structural features.