Second harmonic generation double stokes Mueller polarimetric microscopy of myofilaments | Zendy

Lukas Kontenis | Zendy; Masood Samim | Zendy; Abiramy Karunendiran | Zendy; Serguei Krouglov | Zendy; Bryan A. Stewart | Zendy; Virginijus Barzda | Zendy

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Second harmonic generation double stokes Mueller polarimetric microscopy of myofilaments

Author(s) -

Lukas Kontenis,

Masood Samim,

Abiramy Karunendiran,

Serguei Krouglov,

Bryan A. Stewart,

Virginijus Barzda

Publication year - 2016

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.7.000559

Subject(s) - polarimetry , microscopy , optics , second harmonic generation , myofilament , materials science , physics , biophysics , biology , scattering , laser , myosin

The experimental implementation of double Stokes Mueller polarimetric microscopy is presented. This technique enables a model-independent and complete polarimetric characterization of second harmonic generating samples using 36 Stokes parameter measurements at different combinations of incoming and outgoing polarizations. The degree of second harmonic polarization and the molecular nonlinear susceptibility ratio are extracted for individual focal volumes of a fruit fly larva wall muscle.

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