Open AccessSecond harmonic generation double stokes Mueller polarimetric microscopy of myofilaments
Author(s) -
Lukas Kontenis,
Masood Samim,
Abiramy Karunendiran,
S. I. Kruglov,
Bryan A. Stewart,
Virginijus Barzda
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.000559
Subject(s) - polarimetry , second harmonic generation , stokes parameters , microscopy , optics , polarization (electrochemistry) , myofilament , characterization (materials science) , nonlinear system , materials science , physics , chemistry , biophysics , scattering , biology , laser , myosin , quantum mechanics
The experimental implementation of double Stokes Mueller polarimetric microscopy is presented. This technique enables a model-independent and complete polarimetric characterization of second harmonic generating samples using 36 Stokes parameter measurements at different combinations of incoming and outgoing polarizations. The degree of second harmonic polarization and the molecular nonlinear susceptibility ratio are extracted for individual focal volumes of a fruit fly larva wall muscle.