Fast interferometric second harmonic generation microscopy | Zendy

Stéphane Bancelin | Zendy; CharlesAndré Couture | Zendy; Katherine Légaré | Zendy; Maxime Pinsard | Zendy; Maxime Rivard | Zendy; Cameron Brown | Zendy; François Légaré | Zendy

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Fast interferometric second harmonic generation microscopy

Author(s) -

Stéphane Bancelin,

CharlesAndré Couture,

Katherine Légaré,

Maxime Pinsard,

Maxime Rivard,

Cameron Brown,

François Légaré

Publication year - 2016

Publication title -

biomedical optics express

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.7.000399

Subject(s) - microscopy , optics , interferometry , second harmonic generation , multiphoton fluorescence microscope , interference microscopy , super resolution microscopy , computer science , fluorescence microscope , physics , scanning confocal electron microscopy , laser , fluorescence

We report the implementation of fast Interferometric Second Harmonic Generation (I-SHG) microscopy to study the polarity of non-centrosymmetric structures in biological tissues. Using a sample quartz plate, we calibrate the spatially varying phase shift introduced by the laser scanning system. Compensating this phase shift allows us to retrieve the correct phase distribution in periodically poled lithium niobate, used as a model sample. Finally, we used fast interferometric second harmonic generation microscopy to acquire phase images in tendon. Our results show that the method exposed here, using a laser scanning system, allows to recover the polarity of collagen fibrils, similarly to standard I-SHG (using a sample scanning system), but with an imaging time about 40 times shorter.

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