Open AccessFast interferometric second harmonic generation microscopy
Author(s) -
Stéphane Bancelin,
Charles-André Couture,
Katherine Légaré,
Maxime Pinsard,
Maxime Rivard,
Cameron Brown,
François Légaré
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.000399
Subject(s) - optics , second harmonic generation , interferometry , lithium niobate , microscopy , materials science , laser , phase (matter) , laser scanning , high harmonic generation , optical microscope , scanning electron microscope , physics , quantum mechanics
We report the implementation of fast Interferometric Second Harmonic Generation (I-SHG) microscopy to study the polarity of non-centrosymmetric structures in biological tissues. Using a sample quartz plate, we calibrate the spatially varying phase shift introduced by the laser scanning system. Compensating this phase shift allows us to retrieve the correct phase distribution in periodically poled lithium niobate, used as a model sample. Finally, we used fast interferometric second harmonic generation microscopy to acquire phase images in tendon. Our results show that the method exposed here, using a laser scanning system, allows to recover the polarity of collagen fibrils, similarly to standard I-SHG (using a sample scanning system), but with an imaging time about 40 times shorter.