Open AccessQuantitative spatial frequency fluorescence imaging in the sub-diffusive domain for image-guided glioma resection
Author(s) -
Mira Sibai,
Israël Veilleux,
Jonathan T. Elliott,
Frédéric Leblond,
Brian C. Wilson
Publication year - 2015
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.6.004923
Subject(s) - protoporphyrin ix , autofluorescence , fluorescence , ex vivo , fluorescence lifetime imaging microscopy , biomedical engineering , glioma , optics , materials science , in vivo , nuclear magnetic resonance , medicine , physics , cancer research , biology , microbiology and biotechnology
Intraoperative 5- aminolevulinic acid induced-Protoporphyrin IX (PpIX) fluorescence guidance enables maximum safe resection of glioblastomas by providing surgeons with real-time tumor optical contrast. However, visual assessment of PpIX fluorescence is subjective and limited by the distorting effects of light attenuation and tissue autofluorescence. We have previously shown that non-invasive point measurements of absolute PpIX concentration identifies residual tumor that is otherwise non-detectable. Here, we extend this approach to wide-field quantitative fluorescence imaging by implementing spatial frequency domain imaging to recover tissue optical properties across the field-of-view in phantoms and ex vivo tissue.