Color-coded LED microscopy for multi-contrast and quantitative phase-gradient imaging | Zendy

Donghak Lee | Zendy; Suho Ryu | Zendy; Uihan Kim | Zendy; Daeseong Jung | Zendy; Chulmin Joo | Zendy

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Color-coded LED microscopy for multi-contrast and quantitative phase-gradient imaging

Author(s) -

Donghak Lee,

Suho Ryu,

Uihan Kim,

Daeseong Jung,

Chulmin Joo

Publication year - 2015

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.6.004912

Subject(s) - microscopy , phase contrast microscopy , optics , phase contrast imaging , phase imaging , contrast (vision) , image processing , materials science , computer science , artificial intelligence , physics , image (mathematics)

We present a multi-contrast microscope based on color-coded illumination and computation. A programmable three-color light-emitting diode (LED) array illuminates a specimen, in which each color corresponds to a different illumination angle. A single color image sensor records light transmitted through the specimen, and images at each color channel are then separated and utilized to obtain bright-field, dark-field, and differential phase contrast (DPC) images simultaneously. Quantitative phase imaging is also achieved based on DPC images acquired with two different LED illumination patterns. The multi-contrast and quantitative phase imaging capabilities of our method are demonstrated by presenting images of various transparent biological samples.

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