Open AccessColor-coded LED microscopy for multi-contrast and quantitative phase-gradient imaging
Author(s) -
Donghak Lee,
S. Ryu,
Uihan Kim,
Daeseong Jung,
Chulmin Joo
Publication year - 2015
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.6.004912
Subject(s) - optics , contrast (vision) , microscope , microscopy , phase contrast microscopy , bright field microscopy , artificial intelligence , materials science , phase (matter) , computer vision , light emitting diode , differential interference contrast microscopy , computer science , physics , quantum mechanics
We present a multi-contrast microscope based on color-coded illumination and computation. A programmable three-color light-emitting diode (LED) array illuminates a specimen, in which each color corresponds to a different illumination angle. A single color image sensor records light transmitted through the specimen, and images at each color channel are then separated and utilized to obtain bright-field, dark-field, and differential phase contrast (DPC) images simultaneously. Quantitative phase imaging is also achieved based on DPC images acquired with two different LED illumination patterns. The multi-contrast and quantitative phase imaging capabilities of our method are demonstrated by presenting images of various transparent biological samples.