Open AccessHollow core photonic crystal fiber for monitoring leukemia cells using surface enhanced Raman scattering (SERS)
Author(s) -
Altaf Khetani,
Ali Momenpour,
Emilio I. Alarcon,
Hanan Anis
Publication year - 2015
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.6.004599
Subject(s) - myeloid leukemia , materials science , bone marrow , raman scattering , raman spectroscopy , leukemia , photonic crystal fiber , laser , optical fiber , photonic crystal , optoelectronics , nanotechnology , cancer research , optics , medicine , pathology , immunology , wavelength , physics
The present paper demonstrates an antibody-free, robust, fast, and portable platform for detection of leukemia cells using Raman spectroscopy with a 785-nm laser diode coupled to a hollow core photonic crystal (HC-PCF) containing silver nanoparticles. Acute myeloid leukemia is one of the most common bone marrow cancers in children and youths. Clinical studies suggest that early diagnosis and remission evaluation of myoblasts in the bone marrow are pivotal for improving patient survival. However, the current protocols for leukemic cells detection involve the use of expensive antibodies and flow cytometers. Thus, we have developed a new technology for detection of leukemia cells up to 300 cells/ml using a compact fiber HC-PCF, which offers a novel alternative to existing clinical standards. Furthermore, we were also able to accurately distinguish live, apoptotic and necrotic leukemic cells.