Open AccessModular platform for low-light microscopy
Author(s) -
Tae Jin Kim,
Silvan Tuerkcan,
Andrew Ceballos,
Guillem Pratx
Publication year - 2015
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.6.004585
Subject(s) - microscope , radioluminescence , microscopy , bioluminescence , optics , fluorescence microscope , materials science , biological imaging , multispectral image , bioluminescence imaging , luminescence , optoelectronics , nanotechnology , fluorescence , detector , computer science , chemistry , physics , artificial intelligence , biochemistry , scintillation , transfection , luciferase , gene
Cell imaging using low-light techniques such as bioluminescence, radioluminescence, and low-excitation fluorescence has received increased attention, particularly due to broad commercialization of highly sensitive detectors. However, the dim signals are still regarded as difficult to image using conventional microscopes, where the only low-light microscope in the market is primarily optimized for bioluminescence imaging. Here, we developed a novel modular microscope that is cost-effective and suitable for imaging different low-light luminescence modes. Results show that this microscope system features excellent aberration correction capabilities and enhanced image resolution, where bioluminescence, radioluminescence and epifluorescence images were captured and compared with the commercial bioluminescence microscope.