Open AccessIn vivo longitudinal cellular imaging of small intestine by side-view endomicroscopy
Author(s) -
JoongHo Ahn,
Kibaek Choe,
Taejun Wang,
Yoonha Hwang,
Eunjoo Song,
Ki Hean Kim,
Pilhan Kim
Publication year - 2015
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.6.003963
Subject(s) - endomicroscopy , confocal , confocal microscopy , lumen (anatomy) , gastrointestinal tract , in vivo , pathology , biology , intestinal epithelium , small intestine , preclinical imaging , live cell imaging , microbiology and biotechnology , epithelium , anatomy , medicine , cell , optics , biochemistry , physics , genetics
Visualization of cellular dynamics in the gastrointestinal tract of living mouse model to investigate the pathophysiology has been a long-pursuing goal. Especially, for chronic disease such as Crohn's disease, a longitudinal observation of the luminal surface of the small intestine in the single mouse is highly desirable to investigate the complex pathogenesis in sequential time points. In this work, by utilizing a micro-GRIN lens based side-view endomicroscope integrated into a video-rate confocal microscopy system, we successfully performed minimally-invasive in vivo cellular-level visualization of various fluorescent cells and microvasculature in the small intestinal villi. Also, with a transgenic mouse universally expressing photoconvertible protein, Kaede, we demonstrated repetitive cellular-level confocal endoscopic visualization of same area in the small intestinal lumen of a single mouse, which revealed the continuous homeostatic renewal of the small intestinal epithelium.