Open AccessLabel-free surface-enhanced Raman spectroscopy for detection of colorectal cancer and precursor lesions using blood plasma
Author(s) -
Shouhua Feng,
Wenbo Wang,
Isabella T. Tai,
Guannan Chen,
Rong Chen,
Haishan Zeng
Publication year - 2015
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.6.003494
Subject(s) - colorectal cancer , adenomatous polyps , surface enhanced raman spectroscopy , raman spectroscopy , cancer , medicine , pathology , gastroenterology , materials science , raman scattering , colonoscopy , optics , physics
Fecal based tests have limited diagnostic values in detecting adenomatous polyps, the precursor lesions to colorectal cancer (CRC). Surface enhanced Raman spectroscopy (SERS) using silver nanoparticles as substrate is a multiplexed analytical technique capable of detecting biomolecules with high sensitivity. This study utilizes SERS to analyze blood plasma for detecting both CRC and adenomatous polyps for the first time. Blood plasma samples are collected from healthy control subjects and patients diagnosed with adenomas and CRC. Using a real-time Raman system, SERS spectra for blood plasma samples are measured in 1 s. The collected SERS spectra are analyzed with partial least squares-discriminant analysis. Classification of normal versus CRC plus adenomatous polyps achieved diagnostic sensitivity of 86.4% and specificity of 80%. The results suggest that blood plasma SERS analysis could be a potential screening test to detect both CRC and adenomas.