Open AccessSimple wavefront correction framework for two-photon microscopy of in-vivo brain
Author(s) -
P. Thilanka Galwaduge,
Sharon Kim,
Lauren E. Grosberg,
Elizabeth M. C. Hillman
Publication year - 2015
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.6.002997
Subject(s) - zernike polynomials , optics , wavefront , spatial light modulator , computer science , signal (programming language) , hadamard transform , physics , adaptive optics , computer vision , quantum mechanics , programming language
We present an easily implemented wavefront correction scheme that has been specifically designed for in-vivo brain imaging. The system can be implemented with a single liquid crystal spatial light modulator (LCSLM), which makes it compatible with existing patterned illumination setups, and provides measurable signal improvements even after a few seconds of optimization. The optimization scheme is signal-based and does not require exogenous guide-stars, repeated image acquisition or beam constraint. The unconstrained beam approach allows the use of Zernike functions for aberration correction and Hadamard functions for scattering correction. Low order corrections performed in mouse brain were found to be valid up to hundreds of microns away from the correction location.