Simple wavefront correction framework for two-photon microscopy of in-vivo brain | Zendy

P. Thilanka Galwaduge | Zendy; Sharon Kim | Zendy; Lauren E. Grosberg | Zendy; Elizabeth M. C. Hillman | Zendy

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Simple wavefront correction framework for two-photon microscopy of in-vivo brain

Author(s) -

P. Thilanka Galwaduge,

Sharon Kim,

Lauren E. Grosberg,

Elizabeth M. C. Hillman

Publication year - 2015

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.6.002997

Subject(s) - wavefront , simple (philosophy) , optics , microscopy , two photon excitation microscopy , preclinical imaging , computer science , in vivo , neuroscience , physics , biology , philosophy , microbiology and biotechnology , epistemology , fluorescence

We present an easily implemented wavefront correction scheme that has been specifically designed for in-vivo brain imaging. The system can be implemented with a single liquid crystal spatial light modulator (LCSLM), which makes it compatible with existing patterned illumination setups, and provides measurable signal improvements even after a few seconds of optimization. The optimization scheme is signal-based and does not require exogenous guide-stars, repeated image acquisition or beam constraint. The unconstrained beam approach allows the use of Zernike functions for aberration correction and Hadamard functions for scattering correction. Low order corrections performed in mouse brain were found to be valid up to hundreds of microns away from the correction location.

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