Open AccessInvestigation of temperature effect on cell mechanics by optofluidic microchips
Author(s) -
Tianhang Yang,
Giovanni Nava,
P. Minzioni,
M. Veglione,
Francesca Bragheri,
Francesca Demetra Lelii,
Rebeca Martínez Vázquez,
Roberto Osellame,
Ilaria Cristiani
Publication year - 2015
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.6.002991
Subject(s) - materials science , microfluidics , temperature control , atmospheric temperature range , constriction , optics , optoelectronics , biophysics , nanotechnology , thermodynamics , physics , medicine , endocrinology , biology
Here we present the results of a study concerning the effect of temperature on cell mechanical properties. Two different optofluidic microchips with external temperature control are used to investigate the temperature-induced changes of highly metastatic human melanoma cells (A375MC2) in the range of ~0 - 35 °C. By means of an integrated optical stretcher, we observe that cells' optical deformability is strongly enhanced by increasing cell and buffer-fluid temperature. This finding is supported by the results obtained from a second device, which probes the cells' ability to be squeezed through a constriction. Measured data demonstrate a marked dependence of cell mechanical properties on temperature, thus highlighting the importance of including a proper temperature-control system in the experimental apparatus.