Open AccessFluorescence and reflectance spectral imaging system for a murine mammary window chamber model
Author(s) -
Hui Min Leung,
Arthur F. Gmitro
Publication year - 2015
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.6.002887
Subject(s) - fluorescence , materials science , fluorescence lifetime imaging microscopy , optics , imaging spectroscopy , laser induced fluorescence , absorbance , fluorescence spectroscopy , biomedical engineering , diffuse optical imaging , spectral imaging , spectroscopy , medicine , physics , tomography , quantum mechanics
A spectral imaging system was developed to study the development of breast cancer xenografts in a murine mammary window chamber model. The instrument is configured to work with either a laser to excite fluorescence or a broadband light source for diffuse reflectance imaging. Two applications were demonstrated. First, spectral imaging of fluorescence signals was demonstrated with a GFP-breast cancer tumor and fluorescein injection. Second, based on the principles of broadband reflectance spectroscopy, the instrument was used to monitor dynamic changes of tissue absorbance to yield tissue oxygenation maps at different time points during tumor progression.