Open AccessCharacterization of a Raman spectroscopy probe system for intraoperative brain tissue classification
Author(s) -
Joannie Desroches,
Michael Jermyn,
Kelvin Mok,
Cédric LemieuxLeduc,
Jeanne Mercier,
Karl St-Arnaud,
Kirk Urmey,
MarieChristine Guiot,
Eric Marple,
Kevin Petrecca,
Frédéric Leblond
Publication year - 2015
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.6.002380
Subject(s) - brain tissue , raman spectroscopy , characterization (materials science) , materials science , laser , in vivo , raman scattering , biomedical engineering , spectroscopy , optics , signal (programming language) , nuclear magnetic resonance , pathology , medicine , computer science , nanotechnology , physics , microbiology and biotechnology , quantum mechanics , biology , programming language
A detailed characterization study is presented of a Raman spectroscopy system designed to maximize the volume of resected cancer tissue in glioma surgery based on in vivo molecular tissue characterization. It consists of a hand-held probe system measuring spectrally resolved inelastically scattered light interacting with tissue, designed and optimized for in vivo measurements. Factors such as linearity of the signal with integration time and laser power, and their impact on signal to noise ratio, are studied leading to optimal data acquisition parameters. The impact of ambient light sources in the operating room is assessed and recommendations made for optimal operating conditions. In vivo Raman spectra of normal brain, cancer and necrotic tissue were measured in 10 patients, demonstrating that real-time inelastic scattering measurements can distinguish necrosis from vital tissue (including tumor and normal brain tissue) with an accuracy of 87%, a sensitivity of 84% and a specificity of 89%.