Open AccessCo-localized confocal Raman spectroscopy and optical coherence tomography (CRS-OCT) for depth-resolved analyte detection in tissue
Author(s) -
Jason R. Maher,
Oranat Chuchuen,
Marcus H. Henderson,
Sang-Hoon Kim,
Matthew T. Rinehart,
Angela D. M. Kashuba,
Adam Wax,
David F. Katz
Publication year - 2015
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.6.002022
Subject(s) - optical coherence tomography , confocal , raman spectroscopy , analyte , optics , materials science , chemical imaging , confocal microscopy , spectroscopy , biomedical engineering , chemistry , medicine , computer science , physics , hyperspectral imaging , artificial intelligence , quantum mechanics
We report the development of a combined confocal Raman spectroscopy (CRS) and optical coherence tomography (OCT) instrument (CRS-OCT) capable of measuring analytes in targeted biological tissues with sub-100-micron spatial resolution. The OCT subsystem was used to measure depth-resolved tissue morphology and guide the acquisition of chemically-specific Raman spectra. To demonstrate its utility, the instrument was used to accurately measure depth-resolved, physiologically-relevant concentrations of Tenofovir, a microbicide drug used to prevent the sexual transmission of HIV, in ex vivo tissue samples.