Open AccessA new strategy for label-free detection of lymphoma cancer cells
Author(s) -
Nicola Martucci,
Ilaria Rea,
Immacolata Ruggiero,
Monica Terracciano,
Luca De Stefano,
Nunzia Migliaccio,
Camillo Palmieri,
Giuseppe Scala,
Paolo Arcari,
Ivo Rendina,
Annalisa Lamberti
Publication year - 2015
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.6.001353
Subject(s) - cancer cell , peptide , cancer research , receptor , lymphoma , cell , circulating tumor cell , materials science , cancer , nanotechnology , chemistry , biology , medicine , immunology , biochemistry , metastasis
In this paper, a new strategy for highly selective and sensitive direct detection of lymphoma cells by exploiting the interaction between a peptide and its B-cell receptor, has been evaluated. In particular, an idiotype peptide, able to specifically bind the B-cell receptor of A20 cells in mice engrafted with A20 lymphoma, has been used as molecular probe. The new detection technique has been demonstrated on a planar crystalline silicon chip. Coverage of 85% of silicon surface and detection efficiency of 8.5 × 10(-3) cells/μm(2) were obtained. The recognition strategy promises to extend its application in studying the interaction between ligands and their cell-surface receptors.