Open AccessMesoscopic in vivo 3-D tracking of sparse cell populations using angular multiplexed optical projection tomography
Author(s) -
Lingling Chen,
Yuriy Alexandrov,
Sunil Kumar,
Natalie Andrews,
Margaret J. Dallman,
Paul M. W. French,
James McGinty
Publication year - 2015
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.6.001253
Subject(s) - optics , projection (relational algebra) , tracking (education) , physics , preclinical imaging , iterative reconstruction , frame rate , image resolution , computer vision , artificial intelligence , computer science , in vivo , biology , algorithm , psychology , pedagogy , microbiology and biotechnology
We describe an angular multiplexed imaging technique for 3-D in vivo cell tracking of sparse cell distributions and optical projection tomography (OPT) with superior time-lapse resolution and a significantly reduced light dose compared to volumetric time-lapse techniques. We demonstrate that using dual axis OPT, where two images are acquired simultaneously at different projection angles, can enable localization and tracking of features in 3-D with a time resolution equal to the camera frame rate. This is achieved with a 200x reduction in light dose compared to an equivalent volumetric time-lapse single camera OPT acquisition with 200 projection angles. We demonstrate the application of this technique to mapping the 3-D neutrophil migration pattern observed over ~25.5 minutes in a live 2 day post-fertilisation transgenic LysC:GFP zebrafish embryo following a tail wound.