Open AccessTechnique for real-time tissue characterization based on scanning multispectral fluorescence lifetime spectroscopy (ms-TRFS)
Author(s) -
Dawei Ma,
Julien Bec,
Dimitris Gorpas,
Diego R. Yankelevich,
Laura Marcu
Publication year - 2015
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.6.000987
Subject(s) - multispectral image , fluorescence , fluorescence spectroscopy , fluorescence lifetime imaging microscopy , optics , materials science , computer science , time resolved spectroscopy , spectroscopy , biomedical engineering , artificial intelligence , physics , medicine , quantum mechanics
We report a novel technique for continuous acquisition, processing and display of fluorescence lifetimes enabling real-time tissue diagnosis through a single hand held or biopsy fiber-optic probe. A scanning multispectral time-resolved fluorescence spectroscopy (ms-TRFS) with self-adjustable photon detection range was developed to account for the dynamic changes of fluorescence intensity typically encountered in clinical application. A fast algorithm was implemented in the ms-TRFS software platform, providing up to 15 Hz continuous display of fluorescence lifetime values. Potential applications of this technique, including biopsy guidance, and surgical margins delineation were demonstrated in proof-of-concept experiments. Current results showed accurate display of fluorescence lifetimes values and discrimination of distinct fluorescence markers and tissue types in real-time (< 100 ms per data point).