Open AccessBiodynamic imaging of live porcine oocytes, zygotes and blastocysts for viability assessment in assisted reproductive technologies
Author(s) -
Ran An,
Chunmin Wang,
John Turek,
Zoltán Macháty,
David D. Nolte
Publication year - 2015
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.6.000963
Subject(s) - blastocyst , zygote , oocyte , live cell imaging , assisted reproductive technology , optical coherence tomography , in vitro fertilisation , biology , reproductive technology , embryo quality , cryopreservation , andrology , embryo , microbiology and biotechnology , embryogenesis , medicine , genetics , infertility , cell , radiology , pregnancy
The success of assisted reproductive technologies relies on accurate assessment of reproductive viability at successive stages of development for oocytes and embryos. The current scoring system used to select good-quality oocytes relies on morphologically observable traits and hence is indirect and subjective. Biodynamic imaging may provide an objective approach to oocyte and embryo assessment by measuring physiologically-relevant dynamics. Biodynamic imaging is a coherence-gated approach to 3D tissue imaging that uses digital holography to perform low-coherence speckle interferometry to capture dynamic light scattering from intracellular motions. The changes in intracellular activity during cumulus oocyte complex maturation, before and after in vitro fertilization, and the subsequent development of the zygote and blastocyst provide a new approach to the assessment of preimplant candidates.