Open AccessImaging of a linear diode bar for an optical cell stretcher
Author(s) -
Kevin B. Roth,
Keith B. Neeves,
Jeffrey A. Squier,
David W. M. Marr
Publication year - 2015
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.6.000807
Subject(s) - optics , microfluidics , bar (unit) , laser diode , diode , cardinal point , materials science , laser , plane (geometry) , computer science , optoelectronics , physics , nanotechnology , geometry , mathematics , meteorology
We present a simplified approach for imaging a linear diode bar laser for application as an optical stretcher within a microfluidic geometry. We have recently shown that these linear sources can be used to measure cell mechanical properties; however, the source geometry creates imaging challenges. To minimize intensity losses and simplify implementation within microfluidic systems without the use of expensive objectives, we combine aspheric and cylindrical lenses to create a 1:1 image of the source at the stretcher focal plane and demonstrate effectiveness by measuring the deformation of human red blood cells and neutrophils.