Open AccessReal-time histology in liver disease using multiphoton microscopy with fluorescence lifetime imaging
Author(s) -
Haolu Wang,
Xiaowen Liang,
Yousuf Mohammed,
James A. Thomas,
Kim R. Bridle,
Camilla A. Thorling,
Jeffrey E. Grice,
Zhi Ping Xu,
Xin Liu,
Darrell H. G. Crawford,
Michael S. Roberts
Publication year - 2015
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.6.000780
Subject(s) - histology , autofluorescence , pathology , indocyanine green , fluorescence lifetime imaging microscopy , biopsy , medicine , fluorescence , optics , physics
Conventional histology with light microscopy is essential in the diagnosis of most liver diseases. Recently, a concept of real-time histology with optical biopsy has been advocated. In this study, live mice livers (normal, with fibrosis, steatosis, hepatocellular carcinoma and ischemia-reperfusion injury) were imaged by MPM-FLIM for stain-free real-time histology. The acquired MPM-FLIM images were compared with conventional histological images. MPM-FLIM imaged subsurface cellular and subcellular histopathological hallmarks of live liver in mice models at high resolution. Additional information such as distribution of stellate cell associated autofluorescence and fluorescence lifetime changes was also gathered by MPM-FLIM simultaneously, which cannot be obtained from conventional histology. MPM-FLIM could simultaneously image and quantify the cellular morphology and microenvironment of live livers without conventional biopsy or fluorescent dyes. We anticipate that in the near future MPM-FLIM will be evaluated from bench to bedside, leading to real-time histology and dynamic monitoring of human liver diseases.