Open AccessNon-contact acoustic radiation force impulse microscopy via photoacoustic detection for probing breast cancer cell mechanics
Author(s) -
Jae Youn Hwang,
Bong Jin Kang,
Changyang Lee,
Hyunhee Kim,
Jinhyoung Park,
Qifa Zhou,
K. Kirk Shung
Publication year - 2014
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.6.000011
Subject(s) - acoustic radiation force , acoustic microscopy , materials science , microscopy , transducer , impulse (physics) , contact mechanics , acoustic streaming , atomic force acoustic microscopy , optics , ultrasound , biomedical engineering , acoustics , magnetic force microscope , ultrasonic sensor , physics , medicine , magnetization , finite element method , quantum mechanics , magnetic field , thermodynamics
We demonstrate a novel non-contact method: acoustic radiation force impulse microscopy via photoacoustic detection (PA-ARFI), capable of probing cell mechanics. A 30 MHz lithium niobate ultrasound transducer is utilized for both detection of phatoacoustic signals and generation of acoustic radiation force. To track cell membrane displacements by acoustic radiation force, functionalized single-walled carbon nanotubes are attached to cell membrane. Using the developed microscopy evaluated with agar phantoms, the mechanics of highly- and weakly-metastatic breast cancer cells are quantified. These results clearly show that the PA-ARFI microscopy may serve as a novel tool to probe mechanics of single breast cancer cells.