Open AccessBiocompatible near-infrared fluorescent nanoparticles for macro and microscopic in vivo functional bioimaging
Author(s) -
Liliang Chu,
Shaowei Wang,
Kanghui Li,
Xi Wang,
Xinyuan Zhao,
Jun Qian
Publication year - 2014
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.5.004076
Subject(s) - fluorescence , nanoparticle , materials science , autofluorescence , preclinical imaging , fluorescence lifetime imaging microscopy , microscopy , near infrared spectroscopy , nanotechnology , confocal microscopy , fluorescence microscope , absorption (acoustics) , in vivo , microscope , biological imaging , biomedical engineering , optics , medicine , physics , microbiology and biotechnology , composite material , biology
Near-infrared (NIR) imaging technology has been widely used for biomedical research and applications, since it can achieve deep penetration in biological tissues due to less absorption and scattering of NIR light. In our research, polymer nanoparticles with NIR fluorophores doped were synthesized. The morphology, absorption/emission features and chemical stability of the fluorescent nanoparticles were characterized, separately. NIR fluorescent nanoparticles were then utilized as bright optical probes for macro in vivo imaging of mice, including sentinel lymph node (SLN) mapping, as well as distribution and excretion monitoring of nanoparticles in animal body. Furthermore, we applied the NIR fluorescent nanoparticles in in vivo microscopic bioimaging via a confocal microscope. Under the 635 nm-CW excitation, the blood vessel architecture in the ear and the brain of mice, which were administered with nanoparticles, was visualized very clearly. The imaging depth of our one-photon microscopy, which was assisted with NIR fluorescent nanoprobes, can reach as deep as 500 μm. Our experiments show that NIR fluorescent nanoparticles have great potentials in various deep-tissue imaging applications.