Open AccessNanoparticle assisted photothermal deformation of individual neuronal organelles and cells
Author(s) -
V. H. Romero,
Zurab Kereselidze,
Waskar Egido,
Emmanuel A. Michaelides,
Fidel Santamarı́a,
Xomalin G. Peralta
Publication year - 2014
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.5.004002
Subject(s) - photothermal therapy , materials science , biophysics , nanotechnology , photothermal effect , organelle , nanoparticle , surface plasmon resonance , colloidal gold , microbiology and biotechnology , biology
Stimulation of the localized surface plasmon of metallic nanoparticles has been shown to be an effective mechanism to induce photothermal damage in biological tissues. However, few studies have focused on single cell or subcellular ablation. Our results show that, upon incubation, gold nanostars are internalized by neurons of acute mouse cerebellar brain slices, clustering inside or close to the nucleus. By stimulating the nanostars' surface plasmon using a femtosecond laser, we show deformation of single nuclei and single cells. Given its precision and extremely localized effect, this is a promising technique for photothermal therapy in areas sensitive to collateral thermal damage such as the nervous system.