Open AccessDepth resolved hyperspectral imaging spectrometer based on structured light illumination and Fourier transform interferometry
Author(s) -
Heejin Choi,
Dushan N. Wadduwage,
Paul Matsudaira,
Peter T. C. So
Publication year - 2014
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.5.003494
Subject(s) - hyperspectral imaging , optics , interferometry , imaging spectrometer , spectral imaging , resolution (logic) , spectrometer , spectral resolution , materials science , image resolution , fourier transform , imaging spectroscopy , fourier transform spectroscopy , full spectral imaging , remote sensing , fourier transform infrared spectroscopy , physics , spectral line , computer science , geology , artificial intelligence , quantum mechanics , astronomy
A depth resolved hyperspectral imaging spectrometer can provide depth resolved imaging both in the spatial and the spectral domain. Images acquired through a standard imaging Fourier transform spectrometer do not have the depth-resolution. By post processing the spectral cubes (x, y, λ) obtained through a Sagnac interferometer under uniform illumination and structured illumination, spectrally resolved images with depth resolution can be recovered using structured light illumination algorithms such as the HiLo method. The proposed scheme is validated with in vitro specimens including fluorescent solution and fluorescent beads with known spectra. The system is further demonstrated in quantifying spectra from 3D resolved features in biological specimens. The system has demonstrated depth resolution of 1.8 μm and spectral resolution of 7 nm respectively.