Open AccessRemote focal scanning optical projection tomography with an electrically tunable lens
Author(s) -
Lingling Chen,
Sunil Kumar,
Douglas J. Kelly,
Natalie Andrews,
Margaret J. Dallman,
Paul M. W. French,
James McGinty
Publication year - 2014
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.5.003367
Subject(s) - lens (geology) , optics , projection (relational algebra) , tomography , image resolution , focal length , materials science , resolution (logic) , sample (material) , computer science , physics , artificial intelligence , algorithm , thermodynamics
We describe a remote focal scanning technique for optical projection tomography (OPT) implemented with an electrically tunable lens (ETL) that removes the need to scan the specimen or objective lens. Using a 4× objective lens the average spatial resolution is improved by ∼46% and the light collection efficiency by a factor of ∼6.76, thereby enabling increased acquisition speed and reduced light dose. This convenient implementation is particularly appropriate for lower magnifications and larger sample diameters where axial objective scanning would encounter problems with speed and stability.