Open AccessWavelet transform fast inverse light scattering analysis for size determination of spherical scatterers
Author(s) -
Derek Ho,
Sang-Hoon Kim,
Tyler K. Drake,
Will J. Eldridge,
Adam Wax
Publication year - 2014
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.5.003292
Subject(s) - scattering , sizing , light scattering , optics , robustness (evolution) , materials science , polystyrene , wavelet transform , mie scattering , wavelet , inverse problem , physics , biological system , computer science , mathematics , chemistry , nuclear magnetic resonance , mathematical analysis , artificial intelligence , biochemistry , organic chemistry , biology , gene , polymer
We present a fast approach for size determination of spherical scatterers using the continuous wavelet transform of the angular light scattering profile to address the computational limitations of previously developed sizing techniques. The potential accuracy, speed, and robustness of the algorithm were determined in simulated models of scattering by polystyrene beads and cells. The algorithm was tested experimentally on angular light scattering data from polystyrene bead phantoms and MCF-7 breast cancer cells using a 2D a/LCI system. Theoretical sizing of simulated profiles of beads and cells produced strong fits between calculated and actual size (r(2) = 0.9969 and r(2) = 0.9979 respectively), and experimental size determinations were accurate to within one micron.