Open AccessRemote diffuse reflectance spectroscopy sensor for tissue engineering monitoring based on blind signal separation
Author(s) -
Pedro MartínMateos,
Sergio Crespo-Garcia,
Marta Ruiz-Llata,
José Ramón Lopez-Fernandez,
José L. Jorcano,
Marcela Del Río,
Fernando Larcher,
Pablo Acedo
Publication year - 2014
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.5.003231
Subject(s) - diffuse reflectance infrared fourier transform , principal component analysis , independent component analysis , diffuse reflection , blind signal separation , signal (programming language) , biomedical engineering , materials science , reflectivity , spectroscopy , optics , computer science , chemistry , medicine , artificial intelligence , telecommunications , physics , biochemistry , channel (broadcasting) , photocatalysis , programming language , catalysis , quantum mechanics
In this study the first results on evaluation and assessment of grafted bioengineered skin substitutes using an optical Diffuse Reflectance Spectroscopy (DRS) system with a remote optical probe are shown. The proposed system is able to detect early vascularization of skin substitutes expressing the Vascular Endothelial Growth Factor (VEGF) protein compared to normal grafts, even though devitalized skin is used to protect the grafts. Given the particularities of the biological problem, data analysis is performed using two Blind Signal Separation (BSS) methods: Principal Component Analysis (PCA) and Independent Component Analysis (ICA). These preliminary results are the first step towards point-of-care diagnostics for skin implants early assessment.