Open AccessThree-dimensional optical coherence micro-elastography of skeletal muscle tissue
Author(s) -
Lixin Chin,
Brendan F. Kennedy,
Kelsey M. Kennedy,
Philip Wijesinghe,
Gavin J. Pinniger,
Jessica R. Terrill,
Robert A. McLaughlin,
David D. Sampson
Publication year - 2014
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.5.003090
Subject(s) - optical coherence tomography , skeletal muscle , elastography , computer science , tendon , mdx mouse , biomedical engineering , anatomy , medicine , dystrophin , radiology , ultrasound
In many muscle pathologies, impairment of skeletal muscle function is closely linked to changes in the mechanical properties of the muscle constituents. Optical coherence micro-elastography (OCME) uses optical coherence tomography (OCT) imaging of tissue under a quasi-static, compressive mechanical load to map variations in tissue mechanical properties on the micro-scale. We present the first study of OCME on skeletal muscle tissue. We show that this technique can resolve features of muscle tissue including fibers, fascicles and tendon, and can also detect necrotic lesions in skeletal muscle from the mdx mouse model of Duchenne muscular dystrophy. In many instances, OCME provides better or additional contrast complementary to that provided by OCT. These results suggest that OCME could provide new understanding and opportunity for assessment of skeletal muscle pathologies.