Open AccessIn vivo quantitative photoacoustic microscopy of gold nanostar kinetics in mouse organs
Author(s) -
Weitao Li,
Xiaolian Sun,
Yu Wang,
Gang Niu,
Xiaoyuan Chen,
Zhiyu Qian,
Liming Nie
Publication year - 2014
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.5.002679
Subject(s) - in vivo , photoacoustic imaging in biomedicine , microscopy , preclinical imaging , spleen , fluorescence microscope , materials science , blood circulation , biomedical engineering , kinetics , biodistribution , fluorescence , biophysics , pathology , chemistry , optics , medicine , biology , physics , microbiology and biotechnology , quantum mechanics , traditional medicine
We developed a high-resolution photoacoustic microscopy (PAM) system with a near-infrared (NIR) laser to noninvasively monitor the distribution of gold nanostar (GNS) in blood vessels, liver and spleen in mice. Photoacoustic images of organs at deep depths were continuously acquired in vivo every 30 minutes after a single dose of GNS by tail vein injection. The experimental results showed that GNS accumulated significantly in both liver and spleen from blood circulation after administration, which was qualitatively validated by fluorescence imaging. Our studies demonstrate that PAM might be potentially used for noninvasive tracing the kinetics of exogenous nanoparticles in biological system.