Open AccessBiomolecule-to-fluorescent-color encoder: modulation of fluorescence emission via DNA structural changes
Author(s) -
Takahiro Nishimura,
Yusuke Ogura,
Kenji Yamada,
Yuko Ohno,
Jun Tanida
Publication year - 2014
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.5.002082
Subject(s) - fluorescence , förster resonance energy transfer , biomolecule , encoder , fluorescence in the life sciences , optics , wavelength , materials science , physics , computer science , nanotechnology , operating system
A biomolecule-to-fluorescent-color (B/F) encoder for optical readout of biomolecular information is proposed. In the B/F encoder, a set of fluorescence wavelengths and their intensity levels are used for coding of a biomolecular signal. A hybridization chain reaction of hairpin DNAs labeled with fluorescent reporters was performed to generate the fluorescence color codes. The fluorescence is modulated via fluorescence resonance energy transfer, which is controlled by DNA structural changes. The results demonstrate that fluorescent color codes can be configured based on two wavelengths and five intensities using the B/F encoder, and the assigned codes can be retrieved via fluorescence measurements.