In vivo two-photon microscopy of the hippocampus using glass plugs | Zendy

Mary Grace M. Velasco | Zendy; Michael J. Levene | Zendy

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In vivo two-photon microscopy of the hippocampus using glass plugs

Author(s) -

Mary Grace M. Velasco,

Michael J. Levene

Publication year - 2014

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.5.001700

Subject(s) - microscopy , multiphoton fluorescence microscope , optics , two photon excitation microscopy , in vivo , preclinical imaging , hippocampus , materials science , light sheet fluorescence microscopy , fluorescence microscope , neuroscience , biology , physics , fluorescence , scanning confocal electron microscopy , microbiology and biotechnology

Two-photon microscopy has been used in conjunction with micro-optics, such as GRIN lenses, to access subcortical structures in the intact mouse brain. In this study, we demonstrate the use of thick glass windows, or plugs, for high-resolution, large field-of-view two-photon imaging of the hippocampus in a live mouse. These plugs are less expensive, yield larger fields-of-view and are simpler to use than GRIN lenses while requiring less tissue removal compared to previous methods based on cortical ablation. To demonstrate the capabilities of our system, we show fluorescence images of dendritic spines in the CA1 region of the hippocampus in THY1-YFP transgenic mice.

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