Open AccessIntravital imaging of mouse colonic adenoma using MMP-based molecular probes with multi-channel fluorescence endoscopy
Author(s) -
Gyungseok Oh,
Su Woong Yoo,
Yebin Jung,
YeonMi Ryu,
Youngrong Park,
SangYeob Kim,
Ki Hean Kim,
Sungjee Kim,
SeungJae Myung,
Euiheon Chung
Publication year - 2014
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.5.001677
Subject(s) - molecular imaging , molecular probe , matrix metalloproteinase , fluorescence lifetime imaging microscopy , endoscope , pathology , fluorescence , mmp9 , medicine , cancer research , chemistry , microbiology and biotechnology , biology , in vivo , radiology , optics , downregulation and upregulation , physics , biochemistry , gene
Intravital imaging has provided molecular, cellular and anatomical insight into the study of tumor. Early detection and treatment of gastrointestinal (GI) diseases can be enhanced with specific molecular markers and endoscopic imaging modalities. We present a wide-field multi-channel fluorescence endoscope to screen GI tract for colon cancer using multiple molecular probes targeting matrix metalloproteinases (MMP) conjugated with quantum dots (QD) in AOM/DSS mouse model. MMP9 and MMP14 antibody (Ab)-QD conjugates demonstrate specific binding to colonic adenoma. The average target-to-background (T/B) ratios are 2.10 ± 0.28 and 1.78 ± 0.18 for MMP14 Ab-QD and MMP9 Ab-QD, respectively. The overlap between the two molecular probes is 67.7 ± 8.4%. The presence of false negative indicates that even more number of targeting could increase the sensitivity of overall detection given heterogeneous molecular expression in tumors. Our approach indicates potential for the screening of small or flat lesions that are precancerous.