Open AccessWide-field in vivo background free imaging by selective magnetic modulation of nanodiamond fluorescence
Author(s) -
Susanta K. Sarkar,
Ambika Bumb,
Xufeng Wu,
Kem A. Sochacki,
Peter Kellman,
Martin W. Brechbiel,
Keir C. Neuman
Publication year - 2014
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.5.001190
Subject(s) - nanodiamond , autofluorescence , preclinical imaging , fluorescence lifetime imaging microscopy , fluorescence , materials science , in vivo , molecular imaging , nanotechnology , optics , diamond , physics , microbiology and biotechnology , composite material , biology
The sensitivity and resolution of fluorescence-based imaging in vivo is often limited by autofluorescence and other background noise. To overcome these limitations, we have developed a wide-field background-free imaging technique based on magnetic modulation of fluorescent nanodiamond emission. Fluorescent nanodiamonds are bright, photo-stable, biocompatible nanoparticles that are promising probes for a wide range of in vitro and in vivo imaging applications. Our readily applied background-free imaging technique improves the signal-to-background ratio for in vivo imaging up to 100-fold. This technique has the potential to significantly improve and extend fluorescent nanodiamond imaging capabilities on diverse fluorescence imaging platforms.